Figure 3

Determination of R _s of BsSMC by gel filtration. The column was calibrated by running standard proteins BSA, catalase, and thyroglobulin over the column, then BsSMC. BsSMC eluted in fraction 14.2, which corresponds to an R _s of 10 nm on the extrapolated curve. In repeated experiments, the average R _s was 10.3 nm [19].