Figure 3
From: Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers

Effect of Rottlerin on HMVEC cell number and viability assessed by direct cell counting and MTT assay, respectively. HMVEC were treated with 20 μM Rottlerin (R20) before adding MTT. a Twenty-four hours later, cell number was determined, and b cell viability was measured as described in "Materials and methods". Results are expressed as % of the control (100%). Values are the average of three separate experiments in quadruplicate and are expressed as mean ± SD. *p < 0.05.