Fig. 2
From: Characterising protein/detergent complexes by triple-detection size-exclusion chromatography
a Elution profile and b molar masses of OmpLA/LDAO complexes. a Excess RI values and voltages of UV and LS detectors are depicted as functions of elution volume. PDCs and protein-free detergent micelles elute as two separate peaks at 9 mL and 11 mL, respectively. Peaks at 3 mL and 7 mL are so-called “system peaks” caused by injected air or shedding of the SEC column. b Excess Rayleigh ratios at a scattering angle of 90° and derived molar masses of OmpLA, LDAO, and the OmpLA/LDAO PDC are plotted versus elution volume. 3 mg/mL OmpLA in 50 mM Tris, 100 mM KCl, 2 mM EDTA, pH 8.3, c det,buffer = 4 mM, c det,sample = 5 mM, flow rate 0.4 mL/min, room temperature (RT)