Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.
Skip to main content
Fig. 2 | Biological Procedures Online

Fig. 2

From: Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods

Fig. 2

Comparison of MoDCs viability and maturation. After isolating monocytes with MACS or EasySep technologies, cells were cultured with GM-CSF and IL-4 during 1 week to allow their differentiation into MoDCs, as described in the Material and Methods section. MoDCs were collected and stained with different makers: 7AAD to assess cell viability (a, b); HLA-DR, a maturation marker (c, d); CD86, a co-stimulatory molecule (e, f). a, c, e– MoDCs derived from monocytes isolated with MACS technology; b, d, f– MoDCs derived from monocytes isolated with EasySep technology. Cells were analyzed by flow cytometry and the presented histograms refer to the population of stained MoDC (dark grey) and unstained control (light grey). A representative histogram of one out of eight different donors is shown

Back to article page

Biological Procedures Online

ISSN: 1480-9222

Contact us