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Table 1 Characteristics of monocytes isolated using the MACS or EasySep immunomagnetic isolation technologies

From: Antitumor Efficacy of Human Monocyte-Derived Dendritic Cells: Comparing Effects of two Monocyte Isolation Methods

 

MACS

EasySep

Isolation Yield (%)a

25.00 ± 3.20

18.87 ± 2.69 *

Purity (%) a

94.98 ± 1.91

90.31 ± 2.50

Median FSC

3.49 × 106 ± 0.04 × 106

3.35 × 106 ± 0.05 × 106 **

Median SSC

4.04 × 106 ± 0.15 × 106

6.58 × 106 ± 0.22 × 106 ****

  1. Values represent the Mean ± SEM values from 8 different donors. aThe isolation yield was calculated dividing the number of cells obtained after isolation by the number of cells prior to isolation (total PBMCs) × 100
  2. FSC – forward scatter; SSC – side scatter
  3. Statistical significance (*p < 0.05, **p < 0.01, ****p < 0.0001, a marginal significance 0.05 < p < 0.1) refers to differences between monocyte isolation using both methods
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Biological Procedures Online

ISSN: 1480-9222

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