Fig. 2
From: Optimization of Immunofluorescent Detection of Bone Marrow Disseminated Tumor Cells
Comparison of antigen retrieval methods in cancer cell-spiked human bone marrow. Permeabilization using 0.5% Triton X-100 compared to heat-mediated antigen retrieval using Citrate-based Antigen Retrieval Solution, Target Retrieval Solution, and EDTA solution. White blood cell (WBC) staining represents a cocktail of mouse WBC antibodies (CD45, CD14, CD34, and CD66b) detected by one anti-mouse secondary antibody. Filled white arrowheads point to true positive staining. Scale bar = 50 μm