Table 1 Comparison of the features of anti-HBs qELISA_v.1 and anti-HBs qELISA_v.2
Anti-HBs qELISA_v.1 | Anti-HBs qELISA_v.2 | |
|---|---|---|
Sample dilution buffer | 1% BSA in PBS, pH 7.2 | 10% BSA, 0.1% skim milk and 0.05% Tween 20 in PBS, pH 7.2 |
Secondary antibody dilution buffer | 1% BSA in PBS, pH 7.2 | 10% Goat Serum in PBS, pH 7.2 |
Washing buffer | PBS-T (PBS, pH 7.2, containing 0.05% Tween 20) | 20 mM sodium acetate buffer, pH 4.0, containing 150 mM sodium chloride and 0.05% Tween 20 |