Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.
Skip to main content

Table 1 Upregulated osteoblastic genes by BMSCs-FS (p25) versus ST2 cells

From: A simple and reliable protocol for long-term culture of murine bone marrow stromal (mesenchymal) stem cells that retained their in vitro and in vivo stemness in long-term culture

Gene name

Gene symbol

Fold

Ossification and matrix molecules

 Alkaline phosphatase, liver/bone/kidney

Alpl

7.9

 Bone gamma carboxyglutamate protein

Bglap

2.1

 Collagen, type I, alpha 1

Col1a1

7.5

 Collagen, type I, alpha 2

Col1a2

4.2

 Fibroblast growth factor receptor 1

Fgfr1

4.4

 Fibroblast growth factor receptor 2

Fgfr2

2.3

 Insulin-like growth factor I receptor

Igf1r

2.2

BMP signaling pathway

 Bone morphogenetic protein 2

Bmp2

74

 Bone morphogenetic protein 3

Bmp3

25.3

 Bone morphogenetic protein 5

Bmp5

2

 Bone morphogenetic protein 6

Bmp6

17.7

 Bone morphogenetic protein receptor, type 1B

Bmpr1b

3.8

 Bone morphogenetic protein 4

Bmp4

2

Extracellular Matrix (ECM) Molecules

 Biglycan

Bgn

2

 Collagen, type II, alpha 1

Col2a1

7.5

 Collagen, type III, alpha 1

Col3a1

2.2

 Collagen, type XIV, alpha 1

Col14a1

2.2

 Collagen, type V, alpha 1

Col5a1

2.6

 Integrin alpha 2

Itga2

10

 Integrin alpha 2b

Itga2b

1.5

 Matrix metallopeptidase 2

Mmp2

17

 Matrix metallopeptidase 8

Mmp8

12

 Matrix metallopeptidase 9

Mmp9

23.7

Osteogenic growth factors

 Colony stimulating factor 1 (macrophage)

Csf1

3

 Fibroblast growth factor 1

Fgf1

2.5

 Fibroblast growth factor 2

Fgf2

2.7

 Growth differentiation factor 10

Gdf10

3.7

 Insulin-like growth factor 1

Igf1

2

 Platelet derived growth factor, alpha

Pdgfa

3.2

Osteogenic transcription factors

 Runt related transcription factor 2

Runx2

4.5

 Sp7 transcription factor 7

Sp7

6.7

  1. Cells were induced into osteoblast differentiation as described in the M&M. Mouse osteogenesis RT2 Profiler™ PCR array with 84 osteogenic genes was performed using the SYBR® Green quantitative PCR method. Each target gene was normalized to a group of reference genes as described by manufacture instruction’s. Up-regulated genes (≥ 2 fold) by BMSCs-FS are represented as fold change over ST2 cells
Back to article page

Biological Procedures Online

ISSN: 1480-9222

Contact us