An Alternative Model of Laser-Induced Stroke in the Motor Cortex of Rats

Boyko, Matthew; Kuts, Ruslan; Gruenbaum, Benjamin F.; Tsenter, Philip; Grinshpun, Julia; Frank, Dmitry; Zvenigorodsky, Vladislav; Melamed, Israel; Brotfain, Evgeni; Zlotnik, Alexander

doi:10.1186/s12575-019-0097-x

Biological Procedures Online

Table 1 Assessment of infarct volume, NSS, body temperature, mortality and BBB breakdown in injured hemisphere. ^a = p < 0.01

From: An Alternative Model of Laser-Induced Stroke in the Motor Cortex of Rats

Groups		BBB n = 10		Infarcted Volume n = 10		Temperature n = 20		Mortality n (%)
Groups		Mean and SEM	Variability%	Mean and SEM %	Variability%	Mean and SEM °C	Variability%	Mortality n (%)
Naïve (n = 20)	1 section	80 ± 15	58	0.5 ± 0.3	191	37.2 °C ± 0.1	59	0%
	2 section	113 ± 20	55	0.6 ± 0.2	86
	3 section	174 ± 33	60	0.4 ± 0.3	209
	4 section	151 ± 22	46	0.6 ± 0.3	187
	5 section	161 ± 33	65	0.4 ± 0.3	250
	6 section	127 ± 15	37	0.6 ± 0.3	149
	Total Hemisphere	134 ± 11	25	0.5 ± 0.1	77
Laser 25jx1 (n = 20)	1 section	76 ± 36	148	1.1 ± 0.4	126	37.3 °C ± 0.1	61	0%
	2 section	83 ± 31	120	0.4 ± 0.3	215
	3 section	315 ± 172	172	1.2 ± 0.4	97
	4 section	362 ± 118	103	0.5 ± 0.4	252
	5 section	310 ± 47	48	1.4 ± 0.4	74
	6 section	212 ± 28	42	0.6 ± 0.3	163
	Total Hemisphere	234 ± 67	90	0.9 ± 0.1	49
Laser 50jx1 (n = 20)	1 section	53 ± 43	257	0.5 ± 0.2	142	37.3 °C ± 0.1	55	0%
	2 section	233 ± 90	122	0.9 ± 0.3	116
	3 section	556 ± 180	102	0.7 ± 0.3	115
	4 section	418 ± 88^a	67	0.8 ± 0.2	84
	5 section	281 ± 99	112	0.8 ± 0.3	97
	6 section	326 ± 90^a	88	0.5 ± 0.3	173
	Total Hemisphere	321 ± 63^a	63	0.7 ± 0.1	47
Laser 50jx2 (n = 20)	1 section	89 ± 48	170	0.9 ± 0.2	81	37.3 °C ± 0.1	54	0%
	2 section	308 ± 67^a	69	0.5 ± 0.3	203
	3 section	1072 ± 295^a	87	0.6 ± 0.4	199
	4 section	1147 ± 260^a	72	0.4 ± 0.3	223
	5 section	1262 ± 207^a	52	0.5 ± 0.3	170
	6 section	853 ± 271^a	100	1.1 ± 0.3	87
	Total Hemisphere	725 ± 100^a	44	0.7 ± 0.1	48
Laser 50jx5 (n = 20)	1 section	617 ± 144^a	74	2.3 ± 0.4^a	51	37.4 °C ± 0.1	53	0%
	2 section	854 ± 145^a	53	1.3 ± 0.3	71
	3 section	1114 ± 249^a	71	2.0 ± 0.4^a	59
	4 section	1585 ± 385^a	77	2.8 ± 0.6^a	63
	5 section	2027 ± 460^a	72	1.2 ± 0.3	90
	6 section	1332 ± 340^a	81	1.7 ± 0.5	89
	Total Hemisphere	1255 ± 167^a	42	1.9 ± 0.5^a	33
Laser 50jx10 (n = 20)	1 section	1359 ± 367^a	85	2.2 ± 0.4^a	58	37.4 °C ± 0.1	84	0%
	2 section	2011 ± 431^a	68	2.4 ± 0.5	69
	3 section	1977 ± 318^a	51	3.3 ± 0.5^a	46
	4 section	2632 ± 234^a	28	3.8 ± 0.6^a	51
	5 section	2644 ± 340^a	41	1.6 ± 0.5	92
	6 section	2600 ± 529^a	64	1.3 ± 0.6	162
	Total Hemisphere	2204 ± 280^a	40	2.4 ± 0.3^a	34
t-MCAO (n = 25)	1 section	371 ± 211	179	0.5 ± 0.8	555	38.2 °C ± 0.1^a	121	5 (17%)
	2 section	1053 ± 377^a	113	0.1 ± 0.7	1618
	3 section	1806 ± 507^a	89	6.8 ± 3.0	141
	4 section	2108 ± 262^a	39	2.2 ± 1.3^a	192
	5 section	2649 ± 688^a	89	−0.1 ± 0.8	4625
	6 section	2338 ± 322^a	44	2.2 ± 1	151
	Total Hemisphere	1688 ± 231^a	43	1.9 ± 0.8	123
p-MCAO (n = 24)	1 section	355 ± 147	131	2.6 ± 1.3	164	38.3 °C ± 0.1^a	129	6 (20%)
	2 section	988 ± 200^a	64	9.3 ± 4.7	159
	3 section	3176 ± 750^a	75	19.6 ± 6.6^a	106
	4 section	5213 ± 484^a	29	16.8 ± 4.6^a	87
	5 section	3910 ± 649^a	52	7.1 ± 3.6	161
	6 section	2942 ± 477^a	51	4.0 ± 2.8	228
	Total Hemisphere	2764 ± 256^a	29	9.9 ± 2.9^a	92

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