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Table 5 Inter-assay precision(intermediate precision)test

From: Development and Validation of Quantitative Real-Time PCR for the Detection of Residual CHO Host Cell DNA and Optimization of Sample Pretreatment Method in Biopharmaceutical Products

Standard(pg/ml)

Mean CT

Standard deviation

C.V.(%)

Day1

Day2

Day3

300

14.50

14.3

14.73

0.190

1.312

30

17.78

17.53

17.9

0.179

1.011

3

21.41

21.12

21.49

0.165

0.772

0.3

25.05

24.83

25.00

0.118

0.471

0.03

28.5

28.21

28.42

0.141

0.497

0.003

32.09

31.76

31.82

0.188

0.591