Fig. 3

Effects of osa-MIR156f on gene expression and SL synthesis. a Expression of the tillering and branching related genes in the D18–11 transgenic rice and the wild type (WT) rice; b SL contents in 3-week seedling stage; c SL content at the stem elongation stage (about 70 days after germination). The data calculations are normalized to the internal rice tubulin gene. The ΔΔCt method was used to calculate the relative gene expression. Error bars indicated the standard deviation (SD). Three independent biological replicates were analyzed (n = 3). Asterisks indicate a significant difference to WT rice based on a t test. *: significant difference compared to WT rice at a 5% level (P < 0.05); **: significant difference compared to WT rice at a 1% level (P < 0.01)