Fig. 3
From: Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris
Confirmation of recombinant wild type and mutant clones screened by PCR. Column 1: Negative control, Column 2: DNA ladder, Column 3: PCR products (784 bp) of wild type, Column 4–6: PCR products (784 bp) of autolytic, proteolytic and mixed variants, respectively