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Fig. 5 | Biological Procedures Online

Fig. 5

From: Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris

Fig. 5

Activation of ocriplasmin by urokinase. Reducing SDS–PAGE illustrating that activation with urokinase is almost complete within 30 min. Ocriplasmin (27 KD) is converted into two fragments upon activation. Column 1: Protein marker, Column 2: Ocriplasmin before activation, Column 3–5: Samples collected 10, 30 and 60 min after addition of 2% urokinase. Column 6–8: Samples collected 10, 30 and 60 min after addition of 5% urokinase

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