Fig. 7
From: A Practical Guide to Rodent Islet Isolation and Assessment Revisited
An example of thapsigargin-induced and cytokine-induced cell death as measured by annexin V and propidium iodide fluorescence. a A brightfield image of healthy mouse islets (top), islets treated overnight with 100 nM thapsigargin to induce ER stress (middle), and islets treated overnight with 5 ng/mL IL-1beta and 10 ng/mL TNF-alpha (cytokines) to induce cell death (bottom). b Annexin V is depicted with green fluorescence and propidium iodide is depicted with red fluorescence (c)