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Fig. 5 | Biological Procedures Online

Fig. 5

From: RAD6 Positively Affects Tumorigenesis of Esophageal Squamous Cell Carcinoma by Regulating Histone Ubiquitination of CCNB1

Fig. 5

RAD6A regulates CCNB1 through pro-transcription histone modifications. A and B Western blot analysis of cell cycle-related proteins; C qRT-PCR was used to detect the correlation between RAD6A and CCNB1 expression in ESCC patients; D and E ChIP was used to determine if suppression of RAD6A altered the level of pro-transcription H2B-Ub in the promoters of CCNB1 genes. ** P < 0.01, *** P < 0.001, vs sh-NC

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