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Fig. 2 | Biological Procedures Online

Fig. 2

From: Establishment and Fractionation of Metastatic Axillary Lymph Node Cell Suspension for Determination of Protein Expression Levels of Nuclear cFOS and Cytosolic TGFβ1 from Breast Cancer Patients

Fig. 2

HeLa cell fractionation model illustrated by time curves after serum treatment for: A nuclear cFOS protein, presented by black circles (●), and B cytosolic TGF β1 protein, presented by black triangles (▲). The extracts were obtained by fractionation of growing HeLa cells as described in Methods. All the time points were simultaneously processed and assayed in a single cFOS or TGF-β1 ELISA microplate. Each point was run in duplicate and represents the mean value of two wells. The intra-assay (n = 6) and inter-assay (n = 4) reproducibility was 7 and 15%, respectively

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Biological Procedures Online

ISSN: 1480-9222

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