Table 1 List of specific primers used in this research
Gene | Primer | Sequence 5’–––––––––––3’ | TM(°C) |
|---|---|---|---|
hU6 | Hu6-F | GAGGGCCTATTTCCCATGATT | 62 |
PX459-sgRNA1 | P-LINC001-sg1 | AAGACCTTGTGACTAACCAAC | 62 |
PX459-sgRNA2 | P-LINC001-sg2 | AAGACCCGTCGTTTTTCGAAG | 62 |
LINC00511 | LINC-F LINC-R | CTCCTGTCACAGCCTCAGTG CTCGGCTGAGTCGCGTTC | 62 |
P57 | P57-F P57-R | CTTCTTTGACCCTGACACC CTGAACATGGAGAGATAGTGC | 59 |
Survivin | Sur-F Sur-R | GAGAACGAGCCAGACTTGG GCTTTCCTTTCTGTCAAGAAGC | 62 |
BCL2 | BCL2-F BCL2-R | TGTGGCCTTCTTTGAGTTCG TACAGTTCCACAAAGGCATCC | 58 |
P21 | P21-F P21-R | ACTGTCTTGTACCCTTGTGC CTTCCTCTTGGAGAAGATCAGC | 58 |
Prkca | Prkca-F Prkca-R | ′CGACTGTCTGTAGAAATCTGG CACCATGGTGCACTCCACGTC | 59 |
MDM4 | MDM-F MDM4-R | AATGATGACCTGGAGGACTCTA ACTGCCACTCATCCTCAGAGGTA | 59 |
Map2k6 | Map2k6-F Map2k6-R | AGCGAAACCCTGGCCTTAAA CACACATCACCCTCCCGAAA | 58 |
FADD | FADD-F FADD-R | C GGAAATGGGACAAAACATCCT TGCGGGAGTAGTTGGAAAGT | 59 |
GAPDH | GDH-F GDH-R | GCCAAAAGGGTCATCATCTCTGC GCCAAAAGGGTCATCATCTCTGC | 62 |
E2F1 | E2F1-F E2F1-R | TGATTGTGGCAAAGGAGGA CTCTTCCTTGCTCGTTGTTGGTAT | 62 |