Skip to main content
Fig. 4 | Biological Procedures Online

Fig. 4

From: Circadian gene CSNK1D promoted the progression of hepatocellular carcinoma by activating Wnt/β-catenin pathway via stabilizing Dishevelled Segment Polarity Protein 3

Fig. 4

CSNK1D enhanced the aggressive behaviors and facilitated EMT phenotypes in HCC cells. A The protein and mRNA level of CSNK1D in HCC cell lines was detected by Western blotting and RT-qPCR. B Colony formation assay was conducted to assess cell survival of MHCC97H and HepG2 cells. C The effect of CSNK1D on proliferation of MHCC97H and HepG2 cells was evaluated by performing CCK-8 assay. D Sorafenib sensitivity of HCC cells with CSNK1D knockdown or CSNK1D overexpression. E, F The cell cycle and apoptosis were detected by flowcytometry. G-I The migration activity of MHCC97H and HepG2 cells was assessed by performing wound healing assay and Transwell assay. J Cancer stem cell property was detected by sphere formation assays. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Back to article page

Biological Procedures Online

ISSN: 1480-9222

Contact us