Fig. 4

Alizarin red S test of control and differentiated cells after 7, 14 and 21 days of differentiation. In 21-St. day of differentiation, the Alizarin red S staining test identified calcium deposition. a control (1.00 ± 0.81); b differentiated cells (47.59 ± 3.35) n = 3, (p < 0.001). The control and differentiation samples stained with Alizarin red S after 21 days are depicted