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Fig. 6 | Biological Procedures Online

Fig. 6

From: Ultrasound and microbubble-mediated delivery of miR-424-5p has a therapeutic effect in preeclampsia

Fig. 6

Ultrasound-mediated delivery of miR-424-5p loaded with microbubbles promotes the aggressive phenotype of trophoblast cells. Note: After transfection of lipofection and ultrasonic microbubbles with miR-424-5p overexpression, EdU staining was used to examine the proliferation rate of HTR-8/Svneo and TEV-1 trophoblast cells (A); apoptosis rate of HTR-8/Svneo and TEV-1 trophoblast cells were determined by flow cytometry (B), migration rate by Scratch assay (C) and invasiveness by Transwell invasion assay (D); ELISA examined the levels of PLGF, β-hCG and TNF-α in the supernatant (E). * P < 0.05, **P < 0.01, ***P < 0.001. EdU, 5-Ethynyl-2’-deoxyuridine; PLGF, placental growth factor; β-hCG, human chorionic gonadotropin; TNF-α, tumor necrosis factor-α

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