Fig. 3
Transfer of AMSC-Exo-mediated miR-342-5p into HK-2 cells. Notes: After AMSCs were transfected with LV-miR-342-5p or LV-miR-67, qRT-PCR was used to detect the expression of miR-342-5p in AMSCs (A). The morphology of exosomes was observed by TEM (B), and the particle size of exosomes was measured by Nanosight tracking analysis (C). Western blot was utilized to examine the protein expressions of exosome marker proteins CD9, CD63, and CD81 (D). The mRNA expression of miR-342-5p in AMSC-derived exosomes was measured by qRT-PCR (E). Then, HK-2 cells were subjected to 50 ng/µL of AMSC-Exo, AMSC-Exo-342, or AMSC-Exo-67 treatment for 24 h. qRT-PCR was used to detect the expression of miR-342-5p in HK-2 cells (F). ** P < 0.01, *** P < 0.001, compared to the AMSC-Exo-67 group, N = 3. The red fluorescence in HK-2 cells incubated with DiLC16 (3)-labeled AMSC-Exo-342 was observed under a fluorescence microscope (G); AMSCs, adipose-derived mesenchymal stem cells; Exo, exosome; TEM, transmission electron microscope