Table 4 Detection rates for the three concentrations of the target pathogens
Infectious agent | Target copies/reaction | Detection rate (%) | Target copies/reaction | Detection rate (%) | Target copies/reaction | Detection rate (%) | |
|---|---|---|---|---|---|---|---|
Bacteria | Mycoplasma spp. | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (20/20) 100% |
Leptospira interrogans | 100 | (20/20) 100% | 10 | (16/20) 80% | 1 | (5/20) 25% | |
Brucella canis | 1000 | (20/20) 100% | 100 | (20/20) 100% | 10 | (11/20) 55% | |
Neorickettsia spp. | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (5/20) 25% | |
Rickettsia spp. | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (16/20) 80% | |
Borrelia spp. | 1000 | (20/20) 100% | 100 | (20/20) 100% | 10 | (15/20) 75% | |
Ehrlichia spp. | 100 | (20/20) 100% | 10 | (20/20) 100% | 10 | (14/20) 70% | |
Bartonella spp. | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (7/20) 35% | |
Anaplasma spp. | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (14/20) 70% | |
Protozoan | Leishmania spp. | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (9/20) 45% |
Neospora spp. | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (11/20) 55% | |
Babesia spp. | 1000 | (20/20) 100% | 100 | (20/20) 100% | 10 | (15/20) 75% | |
DNA viruses | CAV-1 | 100 | (20/20) 100% | 10 | (19/20) 95% | 1 | (5/20) 25% |
CaHV-1 | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (14/20) 70% | |
CPV | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (7/20) 35% | |
SuHV-1 | 1000 | (20/20) 100% | 100 | (20/20) 100% | 10 | (13/20) 65% | |
RNA viruses | CIV | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (10/20) 50% |
CPIV | 1000 | (20/20) 100% | 100 | (20/20) 100% | 10 | (20/20) 100% | |
CDV | 1000 | (20/20) 100% | 100 | (20/20) 100% | 10 | (17/20) 85% | |
CCoV | 100 | (20/20) 100% | 10 | (19/20) 95% | 1 | (7/20) 35% | |
Rabies virus | 100 | (20/20) 100% | 10 | (20/20) 100% | 1 | (10/20) 50% |