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Fig. 5 | Biological Procedures Online

Fig. 5

From: Standardized Pre-clinical Surgical Animal Model Protocol to Investigate the Cellular and Molecular Mechanisms of Ischemic Flap Healing

Fig. 5

Vascular separation. For the isolation of SIEV, the largest gap between SIEV and SIEA is identified when the flap is in an extended position (black arrow, A). The needle is inserted from the outside of vessel bundle towards the middle (B). Silk suture is passed (C), cut and tied around SIEV (D). SIEA is similarly separated and isolated by looping the vessel on the opposite side (E)

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