Fig. 3

Murine GFP-positive blastocyst production using the MBCD-SMGE and IVF-IVC methods. A Bright field and (B) fluorescent field images of day 4 mouse blastocysts obtained from the MBCD-SMGE and conventional IVF-IVC methods. In the MBCD-SMGE method, sperm were incubated in the c-TYH medium supplemented with 2 mM MBCD and 20 ng/μl pgRNA-Cas9. C Bright field and (D) fluorescent field images of day 4 mouse negative control blastocysts. Scale bar size: 100 μm