Table 1 miRNA-engineerd EVs as an advanced drug delivery system in Tissue Regeneration Therapies
Therapeutic miRNA | miRNA Enrichment | Incorporation method | Source of EVs | Target | in vitro Cell Lines Utilized | in vitro Findings | in vivo Model | in vivo Findings | Source |
|---|---|---|---|---|---|---|---|---|---|
miR-21-5p | Exogenous | Electroporation | Human ADSCs | Diabetic cutaneous wound healing | HaCaT human keratinocytes | Promotion of cell proliferation and migration via Wnt/b-catenin pathway | Rat diabetic cutaneous wound model | Significant promotion of wound closure, increased re-epithelialization, and increases in both total blood vessel count and mature blood vessel count | Lv, Qijun, et al. [112] |
miR-31-5p | Exogenous | Electroporation | Raw milk | Diabetic wound healing | HUVECs | Increased cell proliferation, migration, and angiogenesis | Rat diabetic cutaneous wound model | Decrease in unclosed wound rate, increased collagen deposition and re-epithelialization rate, and improved vascular network formation | Yan, Chengqi, et al. [113] |
miR-30c | Endogenous | Transfection | Ectopic endometrial endothelial cells | Ovarian endometriosis | N/A | N/A | Murine endometrial model | Decrease in ectopic nodules and attenuation of metastatis via BCL9/Wnt/CD44 regulatory cascade | Zhang, Mengmeng, et al. [114] |
miR-31-5p | Endogenous | Lentiviral Transduction | HEK293T cells | Diabetic wound healing | HaCaT human keratinocytes, HFF-1 human foreskin fibroblast, EA.hy926 human endothelial cells | Promotion of cell proliferation and migration; as well as increased capillary-like construction activity of endothelial cells | Rat diabetic cutaneous wound model | Increases in cutaneous wound closure rate, blood vessel density, and number of mature blood vessels, as well as increased collagen deposition | Huang, Jinghuan, et al. [115] |