Fig. 3
Comparison of DNA yield and amplification success of individual Cx. pipiens pupal exuviae at varying time points post-eclosion. A) Comparison of DNA quantity (ng/µl) from all DNA extractions using the ethanol precipitation method. Horizontal black lines indicate median, 25th and 75th percentiles, whiskers extend to the largest and smallest values within 1.5 times the interquartile range from the 25th and 75th percentiles, closed dots indicate outliers. B) Comparison of PCR amplification success (%) of DNA extracts. Error bars indicate mean ± SD. C) Comparison of nucleic acid concentrations (ng/µl) with mean Cq values for DNA extracts. Grey shaded area indicates the 95% confidence interval. Different letters above the boxplots and bars indicate which groups differ significantly (P < 0.05) from one another