Fig. 5

Comparison of nucleic acid concentration and PCR amplification success of individual field collected Cx. pipiens pupal exuviae between different sampling years. (A) Comparison of nucleic acid concentration (ng/µl) from DNA extracts between different sampling periods using the ethanol precipitation extraction method. Horizontal black lines indicate median, 25th and 75th percentiles, whiskers extend to the largest and smallest values within 1.5 times the interquartile range from the 25th and 75th percentiles, closed dots indicate outliers. (B) Comparison of PCR amplification success (%) for two different sampling periods. Error bars indicate mean ± SD. Different letters above the bars indicate which groups differ significantly (P < 0.05) from one another