Fig. 2

in situ hybridization of Pou5f1/Oct4, Emi2 and cyclin B1 mRNAs in mouse oocytes and embryos. A-C Detection of Pou5f1/Oct4 mRNA in immature oocytes (A-B) and 2-cell stage embryos (C) hybridized with the sense (A) or antisense (B-C) RNA probe. DNA is shown in blue. D The numbers of Pou5f3/Oct4 RNA granules per 100 µm² in individual oocytes and 2-cell stage embryos were counted (means ± standard deviations). Results from three independent experiments were summarized. E-G Detection of Emi2 mRNA in immature oocytes (E-F) and 2-cell stage embryos (G) hybridized with the sense (E) or antisense (F-G) RNA probe. DNA is shown in blue. H The numbers of Emi2 RNA granules per 100 µm² in individual oocytes and 2-cell stage embryos were counted. Results from three independent experiments were summarized. I-K Detection of cyclin B1 mRNA in immature oocytes (I-J) and 2-cell stage embryos (K) hybridized with the sense (I) or antisense (J-K) RNA probe. DNA is shown in blue. L The numbers of cyclin B1 RNA granules per 100 µm² in individual oocytes and 2-cell stage embryos were counted. Results from three independent experiments were summarized. The numbers in parentheses indicate the total numbers of oocytes and embryos analyzed. Statistical significance was analyzed by Student’s t-test. ***p < 0.001. Similar results were obtained from five independent experiments. GC, granulosa cell; GV, germinal vesicle. Bars, 50 μm