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Fig. 3 | Biological Procedures Online

Fig. 3

From: High-sensitivity whole-mount in situ Hybridization of Mouse Oocytes and Embryos Visualizes the Super-resolution Structures and Distributions of mRNA Molecules

Fig. 3

in situ hybridization of Pou5f1/Oct4 mRNA in mouse oocytes and embryos treated with hexanediol or hexanetriol. A Detection of Pou5f1/Oct4 mRNA (green) in immature oocytes (GV) and 2-cell stage embryos (2-cell) without (Control) and with hexanediol or hexanetriol. The oocytes and embryos are outlined by broken lines. DNA is shown in blue. Similar results were obtained from two independent experiments. GV, germinal vesicle. Bars, 50 μm. B The number of RNA granules per 100 µm2 in immature oocytes was counted (means ± standard deviations). C The number of RNA granules per 100 µm2 in 2-cell stage embryos was counted. The numbers in parentheses indicate the total numbers of oocytes and embryos analyzed. Statistical significance was analyzed by the Tukey-Kramer test. *p < 0.05, **p < 0.01. Cont, without treatment; HD, treated with hexanediol; HT, treated with hexanetriol

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Biological Procedures Online

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