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Fig. 4 | Biological Procedures Online

Fig. 4

From: High-sensitivity whole-mount in situ Hybridization of Mouse Oocytes and Embryos Visualizes the Super-resolution Structures and Distributions of mRNA Molecules

Fig. 4

Double fluorescence in situ hybridization of Emi2 (green) and cyclin B1 (red) mRNA in mouse oocytes. A Detection of Emi2 (left) and cyclin B1 (middle) mRNAs by section in situ hybridization. A merged image is shown (Merge). DNA is shown in blue. Note that the signals in granulosa cells would be background of the TSA system in the section in situ hybridization method, which was caused by strong signal amplification, because similar signals were also observed in control sections hybridized with the Emi2 sense RNA probe. B Detection of Emi2 (left) and cyclin B1 (middle) mRNAs by whole-mount in situ hybridization. A merged image is shown (Merge). DNA is shown in blue. C Enlarged views of the boxed region in (B). Similar results were obtained from two independent experiments. GV, germinal vesicle. Bars, 50 μm in A and B, 1 μm in C

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Biological Procedures Online

ISSN: 1480-9222

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