Fig. 5
Double fluorescence in situ hybridization of Pou5f1/Oct4 (green) and cyclin B1 (red) mRNAs in mouse oocytes. A Detection of Pou5f1/Oct4 (left) and cyclin B1 (middle) mRNAs in an immature oocyte by whole-mount in situ hybridization. A merged image is shown (Merge). DNA is shown in blue. B Enlarged views of the boxed regions in (A). C Views of Pou5f1/Oct4 and cyclin B1 mRNAs in an immature oocyte by super-resolution microscopy. A representative top view (X-Y view) is shown on the left. A 3D view (X-Y-Z view) is shown on the right. D An enlarged view of the 25 µm2 square region in (C). E Views of 3D reconstruction of the 25 µm2 square region in (C). Insets are a top view (upper) and a 3D view (lower) of the x-y axis of the 1-µm-thick z-stacks indicated. Similar results were obtained from two independent experiments. Bars, 50 μm in A and C, 1 μm in B, D and E