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Fig. 3 | Biological Procedures Online

Fig. 3

From: USP53 Affects the Proliferation and Apoptosis of Breast Cancer Cells by Regulating the Ubiquitination Level of ZMYND11

Fig. 3

The overexpression and knockdown of USP53 in breast cancer cells. A The expression of USP53 mRNA in several breast cancer cell lines was determined by real-time PCR. Subsequently, USP53 coding sequence and the silencing fragment targeting USP53 was inserted into pcDNA3.1 and pRNAH1.1 vectors, respectively, and these vectors were transfected into MCF-7 and MDA-MB-231 breast cancer cells, respectively. B USP53 mRNA expression was confirmed by real-time PCR after transfection. C, D USP53 protein expression was confirmed by western blot. ***p < 0.001

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Biological Procedures Online

ISSN: 1480-9222

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