Fig. 4

USP53 inhibited the proliferation and cell cycle transition of breast cancer cells. A CCK-8 assay was applied to detect the viability of MCF-7 and MDA-MB-231 cells with USP53 overexpression or silencing. B, C The colony formation ability of MCF-7 and MDA-MB-231 cells was assessed by the colony formation assay. D-F Flow cytometry was performed to determine the cell percentage in each cell cycle phase. G The proliferative index was calculated according to the cell percentage in each phase of flow cytometry results by (G2 + S)/G1. *p < 0.05, **p < 0.01, ***p < 0.001