Fig. 8

The effects of USP53 on breast cancer cells was abolished by ZMYND11 knockdown. A, B The protein level of ZMYND11 after USP53 overexpression or/and ZMYND11 silencing was determined by western blot. C The viability of MCF-7 cells with USP53 overexpression or/and ZMYND11 silencing was assessed by CCK-8 assay. D-F The cell cycle distribution of MCF-7 cells in each phase was detected by flow cytometry and the proliferative index was calculated. G, H The apoptosis of MCF-7 with USP53 overexpression or/and ZMYND11 silencing was examined by Annexin V/PI staining and flow cytometry. I-J The mitochondrial membrane potential of MCF7 cells with transfection of USP53 overexpression plasmid or/and ZMYND11 knockdown vector was determined by JC-1 staining. *p < 0.05, **p < 0.01, ***p < 0.001