Fig. 1

The differentiation of the astrocytic cultures in this study. Overview of the three used protocols based on medium comprised of DMEM-F12 neurobasal medium and supplements media without FBS (LSFP and SSFP) and commercially available basal astrocytes medium (ScienCell) with the addition of FBS during the induction phase (TUSP). Representative bright-field images and immunofluorescence staining images of hiPSC-derived astrocytes expressing GFAP (Green), S100β (blue), and AQP4 (light blue). Scale bars, 50 µm. Pictures were acquired with a Zeiss Apotome 2 microscope. AGS, Astrocyte Growth Supplement (ScienCell); CNTF, Ciliary Neurotrophic Factor; EGF, Epidermal Growth Factor; FGF2, basic fibroblast growth factor 2; LIF, leukemia inhibitory factor; FBS, fetal bovine serum; NPCs, neural precursor cells