Fig. 4

Transcriptomic changes during differentiation of iPSC into mature astrocytes. A Reactome pathway term enrichment analyses were performed for up-regulated genes and down-regulated genes between LSFP and SSFP methods after maturation phase (fold change > 2, base mean > Q3, adjusted p < 0.01). C Reactome pathway term enrichment analyses were also performed for up-regulated genes and down-regulated genes between TUSP and LSFP methods after induction or maturation phase. The same criteria were used for gene selection and statistical significance. B, D The heatmap shows the basemean expression profile of genes in enriched Reactome pathways (FDR < 0.01) for LSFP and AAFP methods (B) and TUSP and LSFP methods (D) after induction (AI) or maturation (AM) phase