Fig. 5

The transcriptome undergoes reprogramming during astrocyte differentiation in the TUSP method using FBS. A The RNA-seq data is presented in a hierarchical clustering and heat map format, displaying all differentially expressed genes between AE-AM samples. The DEGs were divided into two clusters based on their expression patterns. The associated GO categories, KEGG, Reactome and Wiki pathways for each cluster are shown below the heat map. B The ClueGo Cytoscape plug-in was used to perform KEGG and Reactome pathway analysis. A list of unique genes was generated from statistically significant regulated transcripts (> twofold change and Padj < 0.05) among AM3 and AI3 transcript levels. Lists of down-regulated (blue) and up-regulated (red) transcripts were used to query the KEGG and Reactome pathways. The ClueGo parameters were set as follows: Go Term Fusion was selected, only pathways with P-values  < 0.01 were displayed, and a kappa score of 0.40 was used