Fig. 7
The response of the TUSP astrocytes to Aβ42 and oxidative stress. A The Venn diagram showing intersection between the differently expressed transcripts 72 h after treatment of maturated TUSP astrocytes by 500 nM Aβ42 and 200 µM H2O2. B The IL-6 levels in media 72 h after treatment of maturated TUSP astrocytes by 500 nM Aβ42 and 200 µM H2O2. The error bars represent standard error (n = 3), statistical significance was analyzed by Student’s t-test, ** P < 0.01. C, D. The ClueGo Cytoscape plug-in was used to perform GO and Reactome pathway analysis. A list of unique genes was generated from statistically significant regulated transcripts (> twofold change and Padj < 0.01) 72 h after treatment of maturated TUSP astrocytes by 500 nM Aβ42 (D) or 100 µM H2O2 (C). Lists of down-regulated (blue) and up-regulated (red) transcripts were used to query the GO Biology process and function, and Reactome pathways. The ClueGo parameters were set as follows: Go Term Fusion was selected, only pathways with P-values ≤ 0.01 were displayed, and a kappa score of 0.40 was used. E The overrepresented GO biological process classes within astrocyte upregulated transcripts (> twofold change and Padj < 0.01) after treatment of maturated TUSP astrocytes by 200 µM H2O2