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Fig. 5 | Biological Procedures Online

Fig. 5

From: Assessment of MALDI-TOF MS for Arthropod Identification Based on Exuviae Spectra Analysis

Fig. 5

Comparison of exuviae MS spectra from legs as well as those from the thorax + head of triatomines and cockroach. (i) Two exuviae MS spectra of legs (red) and thorax + head (green) from B. germanica (a-d), S. longipalpa (e-h), Tr. infestans (i-l) Rh. prolixus (m-p) are presented. (ii) Principal components analysis (PCA) dimensional image from exuviae MS spectra of B. germanica (A), S. longipalpa (B), Tr. infestans (C) Rh. prolixus (D) comparing legs (red) with those of the thorax + head (green) protein profiles. (iii) LSVs of exuviae leg and those from the thorax + head of the exuviae MS spectra query against homemade MS reference database. The database did not contain thorax + head exuviae MS reference spectra from triatomine or cockroach species. The samples queried were indicated at the bottom of the graphic. The dashed line represents the threshold value (LSV ≥ 1.8), for relevant identification. Comparisons of LSVs done using Wilcoxon matched-pairs signed-rank tests. a.u., arbitrary units; LSVs, log score values; ns, not significant; **, p < 0.01; ***, p < 0.001

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