Assessment of MALDI-TOF MS for Arthropod Identification Based on Exuviae Spectra Analysis

Table 1 Comparison of molecular analysis effectiveness on exuviae samples per arthropod species

Species	Fresh specimens (n = 5)		Exuviae (n = 12)
Species	Stage	Body part^a	Stage	Body part^a	Number of PCR success (%)^b	Number of sequencing success (%)^b	Number of target sequence success (%)^b	Best Match Accession Number (NCBi)
P. humanus corporis	Adults	Whole	3th instars	Whole	0 (-)	/	/	/
Ae. aegypti	Pupae	Whole	Pupae	Whole	0 (-)	/	/	/
An. coluzzii	Pupae	Whole	Pupae	Whole	5 (41.6%)	3 (25.0%)	3 (25%)	MT375223.1
B. germanica	Adults	One leg	≥ 3th instars	Whole	7 (58.3%)	3 (25.0%)	3 (25%)	MT467295.1
S. longipalpa	Adults	One leg	≥ 3th instars	Whole	2 (16.6%)	1 (8.3%)	1 (8.3%)	KU543635.1
Cx. lectularius	Adults	Half^c	4-5th instars	Whole	12 (100%)	3 (25.0%)	1 (8.3%)	MN088697.1
Cx. hemipterus	Adults	Half^c	4-5th instars	Whole	12 (100%)	2 (16.7%)	0 (-)	/
T. infestans	Adults	One leg	4-5th instars	Whole	9 (75.0%)	4 (33.3%)	3 (25.0%)	NC_035547.1
Rh. prolixus	Adults	One leg	4-5th instars	Whole	7 (58.3%)	0 (-)	/	/
Total,n (% )^d	45		108		54 (50.0%)	16 (14.8%)	11 (10,2%)

^aBody part used for DNA extraction. ^bAmong the 12 exuviae per species. ^cThe specimens were cut longitudinally, half was used for DNA extraction and the remaining part was stored at -20 °C. ^dThe proportion among the total number tested, n = 108

ISSN: 1480-9222