Fig. 8

Biological function of ASF1A in HCC. A Expression levels of ASF1A in normal hepatocytes and HCC cell lines. B Western blot analysis demonstrated effective inhibition of ASF1A expression by si-ASF1A-#1 or -#2. C MTT assay revealed that si-ASF1A suppressed the proliferation of Hep G2 and 7721 cells. D Transwell assay showed that si-ASF1A inhibited the migration and invasion abilities of Hep G2 and 7721 cells. E Wound healing assay demonstrated reduced migration and invasion capacities of Hep G2 and 7721 cells upon treatment with si-ASF1A. F In vitro colony formation assay indicated growth inhibition of Hep G2 and 7721 cells by si-ASF1A treatment. Magnification at 100 × , * p < 0.05, ** p < 0.01