Fig. 2

Liver function and liver histopathological changes of mice in each group. (A) Body weight. (B) Liver weight and the liver weight to body weight ratio of the experimental mice at the end of study. (C) Serum levels of ALT, AST and ALP. (D) H&E (left up panel) and Oil Red O (left down panel) staining for hepatic sections representative images of quantification of liver steatosis by assessing the Oil Red O area ratio (right panel) 200×, 100 μm. E NAS scores. Data were expressed as the means ± SD. *P<0.05, **P<0.01 vs. MN group. ^#P<0.05, ^##P<0.01 vs. HN group. FC, fold change, model-to-normal group ratio. MN, standard chow diet; MCDD, methionine-choline-deficient diet; HN, standard chow diet; HFD, high-fat diet