Fig. 1
From: PRRG4 Brain-Specific Conditional Knockout Mice Display Autism Spectrum Disorder-Like Behaviors
Generation of mice with brain-specific conditional knockout of PRRG4 (PRRG4-CKO). A Schematic diagram of knockout PRRG4 (Exon 3) using Emx1-Cre. B Genotyping of PRRG4fl/fl and PRRG4fl/+ mice by conventional PCR. C Genotyping of PRRG4-CKO mice by conventional PCR. D qPCR detection of PRRG4 mRNA levels in the cerebral cortex and hippocampus tissues of PRRG4fl/fl and PRRG4-CKO mice (3 male mice/group). E Immunofluorescence detection of PRRG4 protein levels in the cerebral cortex and hippocampus tissues of PRRG4fl/fl and PRRG4-CKO mice (scale bar: left first column, 200 μm; second to fourth columns, 50 μm). Note: ***: p < 0.001. After generating the PRRG4-CKO mice, we assessed various behavioral, morphological, and molecular characteristics to evaluate the impact of PRRG4 knockout on ASD-like symptoms