Fig. 5

Pyramidal neurons exhibit abnormal dendritic morphology in the cerebral cortex and hippocampus of PRRG4-CKO Mice. A Microscopic photographs of Golgi-stained mouse brain tissue (scale bar: 500 μm). B Representative images of two-dimensional reconstruction of the dendritic trees of pyramidal neurons in cerebral cortex and hippocampus of mice using the Image J plugin (scale bar: 100 μm). C Statistical analysis of the average total dendritic length per pyramidal neuron in the cerebral cortex and hippocampus of mice (3 male mice/group, 6 neurons analyzed per mouse). D Schematic diagram of analyzing the complexity of pyramidal neuron dendritic branching using the Image J plugin. E Statistical analysis of the number of dendritic branches of pyramidal neurons in the cerebral cortex and hippocampus of mice (3 male mice/group, 6 neurons analyzed per mouse). F Representative images of dendritic spine density of Golgi-stained pyramidal neurons in the cerebral cortex and hippocampus of mice (scale bar: 10 μm). G Statistical analysis of the dendritic spine density of pyramidal neurons in the cerebral cortex and hippocampus of mice (3 male mice/group, 12 neuron dendrites analyzed per mouse). Note: ***: p < 0.001, **: p < 0.01, *: p < 0.05, ns: no significant difference