Fig. 7
From: PRRG4 Brain-Specific Conditional Knockout Mice Display Autism Spectrum Disorder-Like Behaviors
PRRG4 interacts with and MAGI2 in SH-SY5Y cell and in the cerebral cortex and hippocampal tissues of PRRG4fl/fl mice. A Immunoprecipitation of PRRG4 in SH-SY5Y cells transduced with empty and PRRG4 overexpression vector using purified PRRG4 antibodies. B Co-immunoprecipitation detection of the interaction between PRRG4 protein and MAGI2 protein in SH-SY5Y cells. Immunoprecipitation was performed using purified PRRG4 antibodies in SH-SY5Y cells transduced with empty and PRRG4 overexpression vector followed by immunoblotting using PRRG4 and MAGI2 antibodies. C Co-immunoprecipitation detection of the interaction between PRRG4 and MAGI2 in mouse brain tissue. Lysates from the cerebral cortex and hippocampus tissues of PRRG4fl/fl and PRRG4-CKO mice were subjected to immunoprecipitation using purified PRRG4 antibodies or normal rabbit IgG as the negative control, followed immunoblotting using PRRG4 and MAGI2 antibodies. D Immunoblotting of MAGI2 content in the cerebral cortex and hippocampus tissues of mice. E Quantitative statistical analysis of MAGI2 protein (6 male mice/group). Note: ns: no significant difference